KMID : 1234420080360040276
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Korean Journal of Microbiololgy and Biotechnology 2008 Volume.36 No. 4 p.276 ~ p.284
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Antifungal Activity of Lactobacillus plantarum Isolated from Kimchi
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Yang Eun-Ju
Chang Hae-Choon
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Abstract
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A lactic acid bacterium having antifungal activity was isolated from kimchi. It was identified as Lactobacillus plantarum based on its morphological and biochemical properties, and 16S rRNA sequence, and designated as Lb. plantarum AF1. This isolate inhibited the growth of Aspergillus flavus ATCC 22546, A. fumigatus ATCC 96918, A. petrakii PF-1, A. ochraceus PF-2, A. nidulans PF-3, Epicoccum nigrum KF-1, and Cladosporium gossypiicola KF-2 under a dual culture overlay assay. Also, the antimicrobial activity was found to be active against various species of Gram-positive and Gram-negative bacteria. The antifungal activity was found to be stable after heat (121oC, 15 min) and proteolytic enzyme treatment, but it was unstable over pH 5.0. The antifungal compound (s) was estimated to have a low molecular mass (below 3,000 Da).
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KEYWORD
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Antifungal activity, kimchi LAB, Lactobacillus plantarum
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